Deleted
Sample Discontinued
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RNA

Part:BBa_K115005:Design

Designed by: Bastiaan van den Berg   Group: iGEM08_TUDelft   (2008-07-29)


Temperature sensitive 5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In contrast to BBa_K115001 a His-tag is added at the 3' end of the thermoswitch. We did this to shift the scar forward, otherwise the scar would alter the original RNA sequence. In case of part BBa_K115001 we left the scar inside the thermoswitch and altered the sequence in such a way that the predicted secundary structure remained the same.


Source

This sequence is taken from the Bradirhizobium Japonicum (BA000040.) as the 5'UTR (ROSE) of a heat shock protein.

References